How it works

NDX developed a product line of versatile cell and tissue acquisition instruments (UnipicK™, UnipicK+™ and A-picK™), which are compatible with a wide range of inverted microscopes.

Our technology is based on the improved aspiration principle that utilizes carefully controlled vacuum pulse to acquire desired tissue region or lift attached cell. Instruments range from manually controlled UnipicK™ to fully automated A-picK™ controlled with our proprietary software, PIKCELLS™.

Microscope compatibility
Instrument configuration Directly integrated Free standing
UnipicK™ Olympus CKX31 / CKX41 / IX73 / IX83 A wide range of microscopes (please contact to ensure compatibility)
UnipicK+™
UnipicK+Auto™ Olympus IX73 / IX83 Olympus IX51/ IX71 / IX81 / IX 53 / IX73 / IX83 NIKON Eclipse MA100
AutopicK™

Advantages

Versatility of our instruments ranges from single cell collection to tissue microdissection, including protocols developed for single cell adhesion force measurement and acquisition of regions of interest from fixed tissue specimens such as formalin fixed paraffin embedded (FFPE) tissues.

The instruments can reliably transfer from nanoliter to microliter volumes and fit multiple models of inverted microscopes using our proprietary universal microscope straddle. Both UnipicK+™ and A-picK™ controlling software offer intuitive and self-explanatory program menus to minimize the learning process and facilitate sample acquisition workflow. The systems are compatible with 96-well workflow and may be used for most single cell analysis protocols. In addition, the instruments are highly adaptable to any protocols offering customizable acquisition, dispensing, detachment, and washing parameters.

Notably, all instruments can collect from any standard culture dishes and microscope glass slides using minimal consumables (DCUs), which can be reused if necessary, minimizing overall cost of experimental work. The instruments including fully automated A-picK™ are the most cost-efficient systems on the market, require minimal routine maintenance and come with a standard three years full warranty.

Summary of advantages

  • Cost-efficiency with minimal consumables
  • Flexible, fits most inverted microscopes
  • High adaptability for customization
  • High viability of collected cells
  • Versatility in applications
  • Functional adhesion test
  • Easy to learn and to use

Representative applications

There are numerous applications in the fields of cancer and stem cell research, neuroscience, developmental biology, -omics (e.g. genomics and proteomics) research, pharmaceutical science, lab-on-a-chip technologies, or basic biology. Any research that requires isolation of single cells or acquisition of subanatomical regions can take advantage of our products. Below are some of the common representative applications. Also, please see our sample videos and representative publications.

Single Cell Biology

Some of the representative publications: Ref1 Ref2 Ref3 Ref4 Ref5

Tissue microdissection:

Some of the representative publications: Ref6 Ref7

If you have questions regarding ordering, delivery, installation and training, please see our FAQ

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Publications

KUDO LC, VI N, MA Z, FIELDS T, AVLIYAKULOV NK, HAYKINSON MJ, BRAGIN A, KARSTEN SL.

Novel Cell and Tissue Acquisition System (CTAS): microdissection of live and frozen brain tissues. PLoS One. 2012;7:e41564.

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MANNELLO F, LIGI D, MAGNANI M.

Deciphering the single-cell omic: innovative application for translational medicine. Expert Rev Proteomics, 2012

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MA Z, KUDO LC, KARSTEN SL.

KuiqpicK™: A Novel Instrument for Rapid Collection of Individual Live Cells from Adherent Cultures. J Biomol Tech. 2014; 25(Suppl): S25.

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XU MJ, COOKE M, STEINMETZ D, KARAKOUSIS G, SAXENA D, BARTLETT E, XU X, HAHN SM, DORSEY JF, KAO GD.

A novel approach for the detection and genetic analysis of live melanoma circulating tumor cells. PLoS One. 2015;10:e0123376.

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LELIÈVRE SA, HODGES, K.B, VIDI P-A.

Application of Theranostics to Measure and Treat Cell Heterogeneity in Cancer (Book Chapter). 2014 Cancer Theranostics

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SURRETTE C, SHOUDY D, CORWIN A, GAO W, ZAVODSZKY MI, KARSTEN SL, MILLER T, GERDES MJ, WOOD N, NELSON JR, PULEO CM.

Microfluidic Tissue Mesodissection in Molecular Cancer Diagnostics. J Lab Autom. 2016.

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KARAKAS HE, KIM J, PARK J, OH JM, CHOI Y, GOZUACIK D, CHO YK.

A microfluidic chip for screening individual cancer cells via eavesdropping on autophagy-inducing crosstalk in the stroma niche. Sci Rep. 2017;7:2050.

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CHEN YC, BAAC HW, LEE KT, FOULADDEL S, TEICHERT K, OK JG, CHENG YH, INGRAM PN, HART AJ, AZIZI E, GUO LJ, WICHA MS, YOON E.

Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano. 2017;11:4660-4668.

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CHIU T-K, ZHAO Y, CHEN D, HSIEH C-H, WANG K, CHOU W-P, LIAO C-J, WANG H-Y, FAN B, WANG J, CHEN J, WU M-H.

A low-sample-loss microfluidic system for the quantification of size-independent cellular electrical property—Its demonstration for the identification and characterization of circulating tumour cells (CTCs). Sensors and Actuators B: Chemical Vol246, 2017, 29–37.

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DORLAND JM.

Variable Response to Chemo therapeutics by a Subpopulation of MCF-7 Breast Cancer Cells. Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, Engineering Honors Undergraduate Thesis, 2016.

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